The Ohio Animal Disease Diagnostic Lab is a full-service, all species animal disease diagnostic facility which provides diagnostic expertise to veterinarians for food animals, horses, small animals, and exotic species. The diagnostic lab also provides increasing support to Ohio's on-farm food safety programs. The laboratory offers >450 different diagnostic tests and completes >500,000 analyses per year. Services offered include avian diagnostics, bacteriology, BSL-3 capabilities, molecular diagnostics, pathology, serology, toxicology, and virology.
The Avian Diagnostics section protects and promotes avian and human health by providing expedient and reliable avian serology services to Ohio's commercial poultry producers, as well as waterfowl, ratite and backyard-exhibition poultry, companion and exotic bird owners.
The majority of the samples received in the Avian Diagnostics section are requests from Ohio commercial chicken and turkey breeder flocks for tests required by the National Poultry Improvement Plan (NPIP). The remainder of the tests completed in this section is associated with either flock profiling programs designed to monitor disease exposure and vaccination response or regulatory testing required for interstate shipments, sales and exhibits.
The Bacteriology section safeguards animal health, the food supply and public health by providing accurate, comprehensive, state-of-the-art, timely, and cost-effective diagnostic services for veterinarians, animal owners, and state and federal regulatory agencies for bacterial and mycotic diseases of livestock and poultry. The Bacteriology section also has a regulatory mission, providing diagnostic support for the National Poultry Improvement Program for control of salmonellosis and mycoplasmosis, trace-back investigations for Salmonella serotype Enteritidis and the trichomonosis control program.
Major services available through the Bacteriology section include the detection, isolation and identification of pathogenic bacteria and fungus from animal tissues, fluids, and environmental samples. Tests offered include aerobic, anaerobic and mycotic culture, Salmonella culture, Johnes culture, antimicrobial susceptibility testing, molecular diagnostics, disease investigation and surveillance, and research, education and outreach activities. The Bacteriology Section has investigated hundreds of cases for the Food and Drug Administration, Ohio Department of Health and pet food companies looking for the presence of Salmonella sp. in dog food.
The ADDL Bacteriology Section has one MALDI-TOF MS instrument for rapid identification of microorganisms:
The Molecular Diagnostics section offers PCR testing and DNA sequencing analysis for screening, diagnosis or confirmation of infectious agents. An average of nearly 30,000 diagnostic molecular tests per year has been performed since 2010. As a member of the National Animal Health Laboratory Network (NAHLN), staff participate in national disease surveillance programs to screen animal populations for reportable diseases including highly pathogenic avian influenza, exotic Newcastle disease, foot and mouth disease, and classical swine fever. Same-day porcine testing is available for PED, porcine deltacoronavirus, PRRS, SIV and Mycoplasma hyopneumoniae. Molecular biology is a progressive and ever evolving field of science; new PCR assays are constantly being introduced, evaluated and implemented at ADDL. DNA sequencing is also performed routinely for identification and confirmation of pathogens.
Major services include:
- Histology - routine H&E and special stains, frozen sections
- Fecal Centrifugation
- Fecal Quantification
- Anaplasmosis(marginale/centrale/ovis) - cELISA
- Bluetongue Virus - AGID, cELISA
- Bovine Leukosis Virus - AGID, ELISA
- Brucella abortus/suis - BAPA, CARD, CF, FPA, SPT, STT
- Brucella canis - Tube Agglutination
- Coxiella burnetii (Q Fever) - CF
- Epizootic Hemorrhagic Disease Virus - AGID
- Equine Infectious Anemia Virus - AGID, ELISA
- Leptospira spp. (pomona, icterohaemorrhagiae, canicola, hardjoprajitno, grippotyphosa, bratislava, sejroe) - MAT
- Mycobacterium paratuberculosis (Johnes) - ELISA, CF
- Ovine Progressive Pneumonia Virus - AGID
- Small Ruminant Lentivirus (CAE/OPP) - cELISA
A multi-laboratory arrangement which includes ODA's Consumer Protection Laboratory (CPL), along with the Chemistry Section of the Diagnostic Laboratory at Iowa State University (ISU), Toxicology section at Texas Veterinary Medical Diagnostic Laboratory, Toxicology section at the Veterinary Medical Diagnostic Laboratory, University of Missouri and The Diagnostic Center for Population and Animal Health at Michigan State University, provide the analytical services. This system has been used for several years.
The Virology section provides high quality diagnostic services to practicing veterinarians, researchers and industry. Major services provided by the Virology section include detection and identification of viruses and viral antigens and/or antibodies from animal tissues, serum, body fluids, and feces. An average of 46,000 diagnostic virology tests per year has been performed since 2010. Virus isolation, fluorescent antibody staining, electron microscopy and aquaculture are standard methods used at ADDL to detect viruses. Virus neutralization, agar gel immunodiffusion, ELISA and hemagglutination inhibition assays are also performed by the Virology section.
The ADDL Virology Section's electron microscope.
Next Generation Sequencing
The Next Generation Sequencing (NGS) section uses an Illumina MiSeq next generation sequencing instrument for detection and identification of new and emerging infectious pathogens. Using NGS, we work with our veterinarians and producers to monitor the status of several pathogens in our herds and flocks by looking at whole genome sequences of bacteria and viruses.