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Lab Sections

Lab Sections

The Ohio Animal Disease Diagnostic Lab is a full-service, all species animal disease diagnostic facility which provides diagnostic expertise to veterinarians for food animals, horses, small animals, and exotic species. The diagnostic lab also provides increasing support to Ohio's on-farm food safety programs. The laboratory offers >450 different diagnostic tests and completes >500,000 analyses per year. Services offered include avian diagnostics, bacteriology, BSL-3 capabilities, molecular diagnostics, pathology, serology, toxicology, and virology.

Avian 

The Avian Diagnostics section protects and promotes avian and human health by providing expedient and reliable avian serology services to Ohio's commercial poultry producers, as well as waterfowl, ratite and backyard-exhibition poultry, companion and exotic bird owners.

The majority of the samples received in the Avian Diagnostics section are requests from Ohio commercial chicken and turkey breeder flocks for tests required by the National Poultry Improvement Plan (NPIP). The remainder of the tests completed in this section is associated with either flock profiling programs designed to monitor disease exposure and vaccination response or regulatory testing required for interstate shipments, sales and exhibits.

Last updated: 7/18/2018

Bacteriology

The Bacteriology section safeguards animal health, the food supply and public health by providing accurate, comprehensive, state-of-the-art, timely, and cost-effective diagnostic services for veterinarians, animal owners, and state and federal regulatory agencies for bacterial and mycotic diseases of livestock and poultry. The Bacteriology section also has a regulatory mission, providing diagnostic support for the National Poultry Improvement Program for control of salmonellosis and mycoplasmosis, trace-back investigations for Salmonella serotype Enteritidis and the trichomonosis control program.

Major services available through the Bacteriology section include the detection, isolation and identification of pathogenic bacteria and fungus from animal tissues, fluids, and environmental samples. Tests offered include aerobic, anaerobic and mycotic culture, Salmonella culture, Johnes culture, antimicrobial susceptibility testing, molecular diagnostics, disease investigation and surveillance, and research, education and outreach activities. The Bacteriology Section has investigated hundreds of cases for the Food and Drug Administration, Ohio Department of Health and pet food companies looking for the presence of Salmonella sp. in dog food.

The ADDL Bacteriology Section has one MALDI-TOF MS instrument for rapid identification of microorganisms:

Last updated: 7/18/2018

Molecular Diagnostics

The Molecular Diagnostics section offers PCR testing and DNA sequencing analysis for screening, diagnosis or confirmation of infectious agents. An average of nearly 30,000 diagnostic molecular tests per year has been performed since 2010. As a member of the National Animal Health Laboratory Network (NAHLN), staff participate in national disease surveillance programs to screen animal populations for reportable diseases including highly pathogenic avian influenza, exotic Newcastle disease, foot and mouth disease, and classical swine fever. Same-day porcine testing is available for PED, porcine deltacoronavirus, PRRS, SIV and Mycoplasma hyopneumoniae. Molecular biology is a progressive and ever evolving field of science; new PCR assays are constantly being introduced, evaluated and implemented at ADDL. DNA sequencing is also performed routinely for identification and confirmation of pathogens. 

Last updated: 7/18/2018

Pathology

The Pathology Section provides necropsy examinations of domestic and wild mammals, birds including poultry, waterfowl, and pet birds, as well as fish, and occasional reptiles and amphibians. Necropsy examinations document morphologic lesions that are associated with disease as the Pathology Section endeavors to establish a definitive cause of illness and cause of death. Some cases involve forensic investigations to determine if abuse or neglect contributed to animals’ deaths. Necropsy examinations also may identify zoonotic infections and thus serve to protect public health, and also confirm the presence of new and emerging diseases in animals. 

The Pathology Section provides comprehensive histopathology services for biopsies, formalin-fixed tissues collected and submitted by practitioners who have conducted their own necropsy examinations, as well as tissues collected by Section pathologists following necropsies conducted at the ADDL. This includes a wide range of special stains as needed to supplement routine histopathology, and an increasing number of immunohistochemistry assays to detect the presence of infectious agent antigens within microscopic lesions. 

The Pathology Section, as an approved laboratory in the National Animal Health Laboratory Network (NAHLN) provides immunohistochemistry testing to detect the prion associated with chronic wasting disease (CWD) in captive and wild cervids, as well as with scrapie in sheep and goats. 

Additional services provided include cytology examinations of smears and fine needle aspirates as well as fecal examinations (centrifugation, flotation and quantitative).

The Pathology Section staff includes four pathologists,  one whom is a board certified diplomate of the American College of Veterinary Pathologists, with cumulative veterinary diagnostic laboratory and necropsy experience surpassing 90 years’ experience.

Last updated: 6/14/2019

Serology

Services:

  • Anaplasmosis(marginale/centrale/ovis) - cELISA
  • Bluetongue Virus - AGID, cELISA
  • Bovine Leukosis Virus - AGID, ELISA
  • Brucella abortus/suis - BAPA, CARD, CF, FPA, SPT, STT
  • Brucella canis - Tube Agglutination
  • Coxiella burnetii (Q Fever) - CF
  • Epizootic Hemorrhagic Disease Virus - AGID
  • Equine Infectious Anemia Virus - AGID, ELISA
  • Leptospira spp. (pomona, icterohaemorrhagiae, canicola, hardjoprajitno, grippotyphosa, bratislava, sejroe) - MAT
  • Mycobacterium paratuberculosis (Johnes) - ELISA, CF
  • Ovine Progressive Pneumonia Virus - AGID
  • Small Ruminant Lentivirus (CAE/OPP) - cELISA

Last updated: 7/18/2018

Toxicology

The Ohio ADDL provides access to toxicology expertise through an ABVT toxicologist available for consultation, analytical test recommendation and interpretation.  Analysis of samples either submitted or acquired through necropsy by staff in the Pathology Section are then shipped to one of several appropriate laboratories for chemical analysis, utilizing a network of laboratories accredited by the American Association of Veterinary Laboratory Diagnosticians (AAVLD). Analytical laboratories typically used are California Animal Health and Food Safety Laboratory, the Chemistry Section of the Diagnostic Laboratory at Iowa State University (ISU), Toxicology section at Texas Veterinary Medical Diagnostic Laboratory, Toxicology section at the Veterinary Medical Diagnostic Laboratory, University of Missouri or The Diagnostic Center for Population and Animal Health at Michigan State University. This approach has been used for years and allows an extensive range of analytical tests to be available for individual cases.  Note that specific laboratory fees and associated shipping charges are case related charges.

Last updated: 6/14/2019

Virology

The Virology section provides high quality diagnostic services to practicing veterinarians, researchers and industry. Major services provided by the Virology section include detection and identification of viruses and viral antigens and/or antibodies from animal tissues, serum, body fluids, and feces. An average of 46,000 diagnostic virology tests per year has been performed since 2010. Virus isolation, fluorescent antibody staining, electron microscopy and aquaculture are standard methods used at ADDL to detect viruses. Virus neutralization, agar gel immunodiffusion, ELISA and hemagglutination inhibition assays are also performed by the Virology section. 

The ADDL Virology Section's electron microscope.

Last updated: 7/18/2018

Next Generation Sequencing

The Next Generation Sequencing (NGS) section uses the Illumina MiSeq and iSeq next generation sequencing instruments for detection and identification of new and emerging infectious pathogens. Using NGS, we work with our veterinarians and producers to monitor the status of several pathogens in our herds and flocks by looking at whole genome sequences of bacteria and viruses. The ADDL is part of the FDA Vet-LIRN and FDA GenomeTrakr network of labs that utilize whole genome sequencing for pathogen identification. In 2019, the laboratory received ISO 17025 accreditation of its method for whole genome sequencing of bacterial pathogens.

Pictured above, top row L to R: Melanie Prarat, Katie Shiplett, Dominika Jurkovic; bottom row L to R: Mary Beth Weisner, Yan Zhang, Jing Cui

Last updated: 9/13/2019