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PRRSV Sequencing Updates: Now Reporting Lineage

Porcine reproductive and respiratory syndrome virus (PRRSV) is the most economically important pathogen endemic in US swine. Did you know that the ADDL offers sequencing of the ORF5 region of PRRSV with a turnaround time of 3-5 days? ORF5 is a highly variable region encoding the PRRSV major envelope protein (GP5). Sequence analysis of this region can provide insight into genetic variation for monitoring distribution, emerging variants, and changes within herds. PRRSV can be classified into one of 9 lineages (L1-L9). Our PRRSV ORF5 sequencing reports include restriction fragment length polymorphism (RFLP) analysis, data regarding relatedness to vaccine strains, and phylogenetic analysis upon request. The ADDL will now also include PRRSV lineage. Descriptions of each lineage may be found on the Swine Disease Reporting System (SDRS) webpage (ISU PRRSV Lineage Descriptions).       

The SDRS was established to share information on swine diseases in the USA, helping animal health professionals make informed decisions about disease prevention, detection, and management. After its establishment, the initial focus of the SDRS network was porcine reproductive and respiratory syndrome virus (PRRSV) reporting, but now the scope has grown to include swine influenza and swine enteric coronaviruses, among others. The network is a collaboration between the Iowa State VDL, University of Minnesota VDL, South Dakota ADRDL, Kansas State VDL, and Ohio ADDL.

Ashley Sawyer, M.S., Virology Researcher