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Ohio Department of Agriculture | Animal Disease Diagnostic Laboratory

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CategoryFeeTypeAmountDescription
OPDNBase fee$30.00Per sample. The base fee includes microscopic examination of sample and moist chambering/basic culturing on media.
OPDNCloning and sequencing PCR products$100.00Per PCR product to be cloned. Squencing will be an additional fee of up to ten dollars per reaction. Fee includes amplification using specific primers, DNA purification, PCR products ligated into a plasmid vector, transformation of E. coli cells, and scre
OPDNDouble-stranded ribonucleic acid (dsRNA) analysis$60.00Per sample. Fee includes dsRNA extraction, purification, and electrophoretic analysis.
OPDNEnzyme-linked immunosorbent assay (ELISA) for common pathogens$50.00Per tissue sample. Fee includes sample preparation, sample test in duplicate wells, and spectrophotometric evaluation of test wells. Fee is applicable to common pathogens and does not apply to mycotoxin or pesticide ELISA tests.
OPDNFatty acid methyl esterase (FAME)/ Gas chromatography (GC)$60.00Per sample. Fee includes sample preparation and GC analysis.
OPDNImmunocapture reverse-transcription PCR (IC-RT-PCR)$70.00Per sample. Fee includes immunocapture with antibody-conjugated magnetic beads, cDNA synthesis by reverse transcription, PCR reaction, and electrophoretic analysis.
OPDNNon-SCN nematode test$30.00Per tissue or soil sample. Fee includes extraction by Baermann funnel or pie plate method, and nematode count.
OPDNPolymerase chain reaction (PCR)$60.00Per sample. Fee includes DNA extraction, purification, PCR reaction, and electrophoretic analysis.
OPDNQuantitative ELISA for mycotoxins$60.00Per grain or tissue samples. Fee includes sample preparation, sample test in single test well plus known standards, and spectrophotmetric evaluation of test wells.
OPDNReal time PCR for detection of specific pathogens$75.00Per sample.
OPDNRT-PCR using cDNA from dsRNA or total RNA template$125.00Per sample. Fee includes dsRNA/total RNA extraction/purification, cDNA synthesis by reverse transcription, PCR reaction, and electrophoretic analysis.
OPDNSoybean cyst nematode (SCN) test$25.00For processing a soil sample for SCN. The fee includes cyst and/or egg counts.
OPDNUnknown fungi ITS real time PCR assay$80.00Per sample. Fee includes DNA extraction, ITS region amplification by real time PCR, DNA sequencing, and sequence results subjected to a BLASTn database search.
OPDNVirus detection/identification by partial purification and transmission electron microscopy (TEM)$80.00Per sample. Fee includes partial virus purification from plant tissue, negative staining preparation, and TEM evaluation.